MOLECULAR STRUCTURE: soluble 94 kd dimeric fusion protein consisting of mouse IL-2 fused to mutant mouse Fcg2a Fc

TRANSFECTANT CELL LINE: NS.1 cells

INFORMATION: Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation (1). It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophilis, and induces mononuclear cells to secrete gamma interferon and tumor necrosis factors alpha and beta (1-4). Moreover, recent studies have shown that IL-2 is required foractivation induced apoptosis, an important hemeostatic mechanism in the immune system, which involve in the maintenance of peripheral tolerance to self-antigens (5, 6). A mouse IL-2/Fc fusion protein is made by genetically fusing IL-2 to Fcg2a. This fusion protein possesses both the biological functions of the IL-2 moiety and a prolonged circulating half-life determined by the Fc domain. Mutations to the complement (C1q) and FcgR I binding sites of the Fcg2a fragment render IL-2/Fc incapable to direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC) (7). This reagent is used successfully in basic and applied research (8 -10).

STORAGE CONDITIONS: Store stock solution at -20oC. Store working solution at 4oC. Freeze/Thawing is not recommended.

PRODUCT STABILITY: Product should retain for at least one year after shipping date when stored at -20oC and the working solution should retain for at least one week at 4oC.

BUFFER: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150 mM NaCl.

PRODUCTION: Mouse IL-10/Fc fusion protein was purified from serum free tissue culture supernatant of CHO transfectants. Purity was >99% by SDS-PAGE.

*This Product is intended for Laboratory Research use only.

MOLECULAR STRUCTURE:A soluble 98 kd dimeric fusion protein consisting of mouse IL-10 fused to mutant mouse Fcg2a Fc.

TRANSFECTANT CELL LINE:CHO cells

INFORMATION:Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages (1). In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells, and monocytes/macrophages (1). Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock (2) and as an immunosuppressive agent in certain T-cell mediated autoimmune diseases (3, 4). A mouse IL-10/Fc fusion protein is made by genetically fusing IL-10 to Fcy2a. This fusion protein possesses both the biological functions of the IL-10 moiety and a prolonged circulating half-life determined by the Fc domain. Mutations to the complement (C1q) and FcyR I binding sites of the Fcy2a fragment render IL-10/Fc incapable to direct antibody directed cytotoxicity (ADCC) and compliment directed cytotoxicity (CDC) (5).

STORAGE CONDITIONS:Store stock solution at -20oC. Store working solution at 4oC. Freeze/Thawing is not recommended.

PRODUCT STABILITY:Product should retain for at least one year after shipping date when stored at -20oC and the working solution should retain for at least one week at 4oC.

BUFFER:50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150 mM NaCl.

PRODUCTION:Mouse IL-10/Fc fusion protein was purified from serum free tissue culture supernatant of CHO transfectants. Purity was >99% by SDS-PAGE. The endotoxin level is < 0.06 EU per mg of IL-10/Fc.

*This Product is intended for Laboratory Research use only

MOLECULAR STRUCTURE:A soluble 110 kd dimeric fusion protein consisting of human IL-2 fused to human IgG3 Fc.

TRANSFECTANT CELL LINE:NS1 cells

INFORMATION:Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation (1). It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophilis, and induces mononuclear cells to secrete gamma interferon and tumor necrosis factors alpha and beta. Moreover, recent studies have shown that IL-2 is required for activation induced apoptosis, an important hemeostatic mechanism in the immune system, which involve in the maintenance of peripheral tolerance to self-antigens. A human IL-2/Fc fusion protein is made by genetically fusing IL-2 to IgG3 Fc. This fusion protein possesses both the biological functions of the IL-2 moiety and a prolonged circulating half-life determined by the Fc domain.

STORAGE CONDITIONS:Store stock solution at -20oC. Store working solution at 4oC. Freeze/Thawing is not recommended.

PRODUCT STABILITY:Product should retain for at least one year after shipping date when stored at -20oC and the working solution should retain for at least one week at 4oC.

BUFFER:50 mM Sodium Phosphate pH 7.4, 100 mM Potassium Chloride, 150 mM NaCl.

PRODUCTION:Human IL-2/Fc fusion protein was purified from serum free tissue culture supernatant of NS.1 transfectants. Purity was >99% by SDS-PAGE.

*This Product is intended for Laboratory Research use only.

MOLECULAR STRUCTURE:A soluble 110 kd dimeric fusion protein consisting of human IL-10 fused to mutant human IgG1 Fc

TRANSFECTANT CELL LINE:NS1 cells

INFORMATION:Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells natural killer cells, and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T-cell mediated autoimmune diseases. A human IL-10/Fc fusion protein is made by genetically fusing IL-10 to IgG1 Fc. This fusion protein possesses both the biological functions of the IL-10 moiety and a prolonged circulating half-life determined by the Fc domain.

STORAGE CONDITIONS:Store stock solution at -20oC. Store working solution at 4oC. Freeze/Thawing is not recommended.

PRODUCT STABILITY:Product should retain for at least one year after shipping date when stored at -20oC and the working solution should retain for at least one week at 4oC.

BUFFER:50 mM Sodium Phosphate pH 7.4, 100 mM Potassium Chloride, 150 mM NaCl.

PRODUCTION:Human IL-10/Fc fusion protein was purified from serum free tissue culture supernatant of NS.1 transfectants. Purity was >99% by SDS-PAGE.

*This Product is intended for Laboratory Research use only.

INFORMATION: The dual probe CTLA4/Fc ELISA Kit is a quantitative "sandwich" enzyme immunoassay. A rat IgG antibody specific for mouse CTLA4 has been coated onto the microtiter plates provided in the kit. Test samples or CTLA4/Fc standards are added to the mocrotiter wells and any CTLA4 present binds to the pre-coated IgG antibody. After washing away unbound sample components, an enzyme-linked rabbit IgG specific for mouse IgG2a Fc is added to the wells. A sandwich is thus formed with the CTLA4/Fc bound by the coated anti-CTLA4 antibody and detecting anti-mouse IgG 2a Fc antibody. Thus the dual probe ELISA ensures this assay specific for mouse CTLA4/Fc fusion protein but neither CTLA4 nor IgG2a. Excess enzyme-conjugated IgG is removed by washing and a substrate solution is added to the wells. Color develops in proportion to the amount of CTLA4/Fc present in the sandwich.
In addition to the samples tested, a Standard Curve is prepared using known concentrations of the CTLA4/Fc standard provided in the kit. The optical density of the test samples is compared with the Standard Curve and the CTLA4/Fc concentration in the sample is determined.
Dual probe CTLA4/Fc ELISA ensures this assay specific for mouse CTLA4/Fc fusion protein but neither CTLA4 nor IgG2a.

*This Product is intended for Laboratory Research use only.

MOLECULAR STRUCTURE:Purified anti-mouse CD 94

TRANSFECTANT CELL LINE:Rat IgG2a

INFORMATION:The 18d3 monoclonal antibody reacts with mouse CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells and NKT cells, and plays an important role in adhesion and activation of NK cell lineage. CD94/NKG2 is expressed on Th1 but not Th2 cells and costimulates Th1 effector functions. DBA/2J mice are naturally CD94-deficient and do not express cell-surface CD94/NKG2A receptors, even on neonatal NK cells.

STORAGE CONDITIONS:Store stock solution at -20oC. Store working solution at 4oC. Freeze/Thawing is not recommended.

PRODUCT STABILITY:Product should retain for at least one year after shipping date when stored at -20oC and the working solution should retain for at least one week at 4oC.

BUFFER:Anti-CD94 mAb is supplied as a frozen liquid comprised of 0.22 um sterile-filtered PBS (PH 7.4, 50 mM Sodium Phosphate, 100 mM Potassium Chloride, 150 mM NaCl) and containing no preservatives.

PRODUCTION:Rat anti-mouse CD94 mAb was purified from serum free tissue culture supernatant of hybridoma cells. Purity was >98% by SDS-PAGE. The endotoxin level is < 0.06 EU per ug of mAb.

*This Product is intended for Laboratory Research use only.

MOLECULAR STRUCTURE:Purified anti-human CTLA4 (CD152)

TRANSFECTANT CELL LINE:Mouse IgG2a, clone 2

INFORMATION:Cytolytic T lymphocyte-associated antigen, CTLA-4 or CD152, is a cell surface glycoprotein that is transiently expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) expressed on antigen presenting cells (APC). This interaction appears to deliver a negative regulatory signal to the T cell. While CD28 binds to CD80 and CD86 with lower affinity and deliver positive signal fro T cell activation and proliferation. CD28 and CD152 play important roles in regulating the magnitude and nature of T cell mediated immune response. A monoclonal antibody (20A) has been extensively characterized as being capable of blocking human B7-1/2 binding to CTLA-4.

STORAGE CONDITIONS:Store stock solution at -20oC. Store working solution at 4oC. Freeze/Thawing is not recommended.

PRODUCT STABILITY:Product should retain for at least one year after shipping date when stored at -20oC and the working solution should retain for at least one week at 4oC.

BUFFER:Anti-CTLA4 mAb is supplied as a frozen liquid comprised of 0.22 祄 sterile-filtered PBS (PH 7.4, 50 mM Sodium Phosphate, 100 mM Potassium Chloride, 150 mM NaCl) and containing no preservatives.

PRODUCTION:Mouse anti-human CTLA4 mAb was purified from serum free tissue culture supernatant of hybridoma cells. Purity was >98% by SDS-PAGE. The endotoxin level is < 0.06 EU per ug of mAb.

*This Product is intended for Laboratory Research use only.

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