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Product

CD83 (human):Fc (human) (rec.) (non-lytic)

Product Data

Synonyms :  

BL11,HB15

Properties

Host : 

CHO cell 

Sequence : 

The extracellular domain of human CD83 (NP_004224.1) (Thr20-Ala131) 

is fused to the N-terminus of the Fc region of a mutant human IgG1. 

Molecular Mass:

4KDa (monomer)

Non-lytic:

Acts as a long lasting fusion protein which only binds to the receptor. 

Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins i

ncapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

 

 

 

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

Activity:

Measured by the ability of the immobilized protein 

to support the adhesion of human monocyte-derived dendritic cells.

Shipping and Handling

Formulation : 

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

Human CD83 is a 40-50 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins. CD83 is a primary marker for dendritic cells. It is also found on B cells, neutrophils, monocytes and macrophages. Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells. Membrane CD83 promotes T cell proliferation, particularly of CD8+ cytotoxic T cells. Soluble CD83 is immunosuppressive and blocks T cell activation. On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype. A lack of membrane-expressed CD83 leads to an unusual IL-4/ IL-10 producing CD4+ T cell phenotype.

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