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Product

CD27[TNFRSF7] (human):Fc (human) (rec.)

Product Data

Synonyms :  

T-cell activation antigen CD27; T14; 

Tumor necrosis factor receptor superfamily member 7; S152

Properties

Host : 

CHO cells 

Sequence :

The extracellular domain of human CD27[TNFRSF7] (AAH12160.1)( Thr21- Ile192) is fused to the 

N-terminus of the Fc region of human IgG1.

Molecular Mass:

45 KDa (monomer)

 

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

Activity:

Measured by its ability to inhibit the proliferation of mouse T cells 

induced by CD27 Ligand and anti-CD3. 

Shipping and Handling

Formulation : 

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.

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