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Product

CD40L [CD154] (human):Fc (human) (rec.)

Product Data

Synonyms :  

CD154; Tumor Necrosis Factor Ligand Superfamily Member 5;

TNFSF5; Gp39; HIGM1; IGM; IMD3; TRAP

Properties

Host : 

CHO cell 

Sequence : 

The extracellular domain of human CD40L (NP_000065.1)

(Met113-Leu261) is fused to the 

N-terminus of the Fc portion of human IgG1. 

Molecular Mass:

46 KDa (monomer)

 

 

 

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

Activity:

Measured in a cell proliferation assay using enriched human B cells 

in the presence of IL-4. 

Shipping and Handling

Formulation : 

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

CD40 Ligand (CD40L), renamed TNFSF5 but now also known as CD154, TRAP and gp39, is a 34-39kDa typeII transmembrane glycoprotein that belongs to the TNF superfamily. As with other TNF superfamily members, CD40L will exist as a trimer, both as a membrane bound and soluble form. Multiple mutations and alternate splice forms of CD40L exist, often associated with pathology and leading to truncated or nontrimerizable forms of CD40L. CD40L binds to both CD40 and to integrin αIIbβ3 (CD41). In the cell membrane, it also associates with a unique splice variant of CD28 (CD28i) that may facilitate CD40L signal transduction. CD40L is expressed by monocytes, NK cells, mast cells, basophils, smooth muscle cells, endothelial cells, dendritic cells,activated and resting B cells, plus activated platelets and CD4+ T cells. CD40L ligation of CD40 on dendritic cells (DC) initiates DC maturation and differentiation. CD40L signaling into naive B cells promotes germinal center formation and isotope switching. CD40-CD40L seems to bridge innate and adaptive immune signals.

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