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Product

CSF3 (mouse):Fc (mouse) (rec.)

Product Data

Synonyms :  

Granulocyte Colony-stimulating Factor; G-CSF

Properties

Host : 

CHO cell 

Sequence : 

The extracellular domain of mouse CSF3 (CAA28986.1)

(Val31-Ala208) is fused to the 

N-terminus of the Fc region of mouse IgG2a.  

Molecular Mass:

48 kDa (monomer)

 

 

 

Purity :

≥98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

≤60EU/mg as determined by LAL test.

Activity:

Measured by its ability to inhibit the 

G-CSF-induced proliferation of NFS‑60 

mouse myelogenous leukemia lymphoblast cells.

Shipping and Handling

Formulation : 

Lyophilized from 0.2μm-filtered solution in PBS. 

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

 

Documents
Description

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-α activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage in vitro. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

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