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Product

IGF-1 (human):Fc (human) (rec.)

Product Data

Synonyms :  

IGF-1

Properties

Host : 

CHO cell 

Sequence :

The extracellular domain of human IGF-1(AAA52543.1)(Gly26-Met130) is fused to the 

N-terminus of the Fc region of a human IgG1. 

Molecular Mass:

38KDa (monomer)

 

 

Purity :

>98%by SDS-PAGE under reducing conditions 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

Activity:

Measured in a serum-free cell proliferation assay.

Shipping and Handling

Formulation : 

Lyophilized from 0.2 μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100 μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

Rat insulin-like growth factor-I (IGF-I), a serum polypeptide with growth promoting activity, was isolated from rat serum by a combination of acid/ethanol extraction, affinity chromatography, and a series of reversed phase high performance liquid chromatography, cation exchange, and reversed phase. All peptide fragments produced by chymotrypsin digestion of reduced and carboxymethylated rat IGF-I were amino acid sequenced and compared with the sequence of human IGF-I. Three out of 70 of the rat amino acid residues differed from those of human IGF-I as follows: Asp20----Pro, Ser35----Ile and Ala67----Thr. Purified rat IGF-I cross-reacted with polyclonal anti-human IGF-I antibody 75% as compared to human IGF-I, but it cross-reacted only 3% with monoclonal anti-human IGF-I antibody. Thus, it is possible to monitor the metabolic fate of human IGF-I, when injected into rats, without interference by endogenous rat IGF-I. Rat IGF-I showed 65% activity in the radioreceptor, 28.6% activity in the lipogenesis and 22.5% activity in the free fatty acid release inhibition assays as compared to human IGF-I on a protein quantity basis

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