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Product

CD80 [B7-1] (mouse):Fc (mouse) (rec.)

Product Data

Synonyms : 

B7-1; CD80

Properties

Host : 

CHO cell

Sequence :

The extracellular domain of mouse CD80 

[B7-1] (NP_033985.3) (Asp37 - Lys245) is fused to the 

N-terminus of the Fc region of mouse IgG2a.

Molecular Mass:

50 KDa (monomer)

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

 

Activity:

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells.

 

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

 

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T-and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon γ. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% amino acid identity. Human and mouse B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form theB7-1/B7-2/CD28/CTLA-4 critical binding sites.

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