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Product

IL-4 (human):Fc (human) (rec.) (non-lytic)

Product Data

Synonyms : 

Interleukin-4; B Cell Stimulatory Factor 1; BSF-1

Properties

Host : 

CHO cell

Sequence :

The extracellular domain of human IL-4 (NP_000580)(Met1-Ser153) is fused to the N-terminus of the Fc region of a mutant human IgG1.

Molecular Mass:

43 KDa (monomer)

Non-lytic:

Acts as a long lasting fusion protein which only binds to the receptor. 

Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins 

incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

 

Activity:

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

Interleukin-4 (IL-4) is a cytokine produced by type 2 helper T cells, the Th2 cells. These cells tends to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-γ, and lymphotoxin (TNF-β). In addition, mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunoglobulin class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. IL-4 promotes survival, growth, and differentiation of both T and B lymphocytes, mast cells and endothelial cells. In addition, IL-4 inhibits the production of TNF, IL-1, and IL-6 by macrophages.

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