Manufacturer of Immune Regulation Proteins
Your Position : Home > Products > Interleukin and their receptor Cytokines > IL-21 (human):Fc (human) (rec.) (non-lytic)
Product

IL-21 (human):Fc (human) (rec.) (non-lytic)

Product Data

Synonyms : 

Interleukin-21

Properties

Host :

CHO cell

Sequence :

The extracellular domain of human IL-21 (NP_068575.1) (Gln32-Ser162) is fused to the N-terminus of the Fc region of a mutant human IgG1.

Molecular Mass:

44 KDa (monomer)

Non-lytic:

Acts as a long lasting fusion protein which only binds to the receptor. 

Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins 

incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

Activity:

Measured in a cell proliferation assay using N1186 human T cells. 

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

Interleukin-21 (IL-21) is a key factor in the transition between innate and adaptive immune responses secreted by activated T cells. The IL-21 receptor (IL-21R) is expressed in lymphoid tissue, in particular by NK, B, T and dendritic cells, macrophages and endothelial cells. Recent evidence suggests that IL-21 plays a supportive role in the proliferation of T and B cells and influences the cytolytic activity of natural killer cells. IL-21 has been shown to up-regulate genes associated with innate immunity and to inhibit the differentiation of naïve T helper cells. IL-21 specifically inhibits IFN-γ production from developing TH1 cells and is preferentially expressed by TH2 cells. Furthermore IL-21 has been identified as a growth and survival factor for human myeloma cells. IL-21/IL-21R interactions have a unique role in sequentially activating both innate and adaptive immune responses against poorly immunogenic tumors, leading to

tumor rejection that is perforin dependent but IFN-γ independent.

Online Message